Cov ntaub so ntswg Embedder

Xiaogan Kuohai Medical Technology Co., Ltd

Xiaogan Kuohai Medical Technology Co., Ltd. yog ib lub tuam txhab tsim khoom siv hluav taws xob tsawg tsawg nrog cov neeg siv thoob plaws hauv Suav teb. Nws kuj yog ib qho ntawm ob peb lub tuam txhab hauv kev lag luam hauv tsev thiab thoob ntiaj teb uas muaj peev xwm tshawb nrhiav, tsim kho, thiab ua tiav cov cuab yeej siv tshuaj thiab cov khoom siv. Nyob rau hauv 2014, lub tuam txhab tsim ib tug niaj hnub ntau lawm thiab ua puag.

Vim Li Cas Xaiv Peb

Kev paub nplua nuj
Tom qab xyoo ntawm kev sib sau ua ke hauv kev lag luam kho mob, peb tau nkag mus rau hauv kev lag luam khoom siv kho mob.

Pab neeg tshaj lij
Thaum pib ntawm kev tsim, lub tuam txhab tau teeb tsa lub hom phiaj meej: cov neeg siv khoom-centric, kev tsim kho tshiab

Kev Pabcuam Kev Cai
Kev cog lus los muab cov cuab yeej ua haujlwm zoo thiab cov kev pabcuam pathological, ua rau muaj txiaj ntsig zoo rau kev txhim kho kev lag luam thiab kev vam meej hauv zej zog

One-stop Solution
Kev them nyiaj yug rau cov neeg siv khoom kom ntseeg tau tias kev lag luam du.

Dab tsi yog Tissue Embedder

Cov ntaub so ntswg embedder yog cov cuab yeej kuaj siv hauv cov txheej txheem ntawm histology los embed cov ntaub so ntswg lom nyob rau hauv ib qho nruab nrab, xws li paraffin wax, rau cov ntu txuas ntxiv thiab tshuaj xyuas microscopic. Cov txheej txheem embedding muaj nyob ib puag ncig cov qauv ntaub so ntswg nrog rau qhov nruab nrab embedding los tsim ib qho thaiv uas tuaj yeem yooj yim tuav thiab hlais rau hauv nyias ntu siv microtome.

Cov ntaub so ntswg embedder feem ntau muaj lub hauv paus phaj nrog kev nyuaj siab los yog pwm qhov twg cov embedding nruab nrab yog poured thiab tso cai rau solidify nyob ib ncig ntawm cov ntaub so ntswg qauv. Thaum qhov nruab nrab embedding tau hardened, cov ntaub so ntswg thaiv tau raug tshem tawm ntawm lub embedder thiab khaws cia kom txog thaum nws npaj txhij mus rau seem.

Embedding cov ntaub so ntswg nyob rau hauv paraffin ciab tso cai rau yooj yim tuav thiab manipulation ntawm cov ntaub so ntswg qauv thaum lub sij hawm cov txheej txheem histological. Nws kuj tseem ua rau cov khoom nyias nyias, cov ntu uas tuaj yeem stained thiab pom nyob rau hauv lub tshuab tsom rau kev kuaj xyuas cov qauv ntawm tes thiab kev hloov pauv pathological.

Cov ntaub so ntswg embedders feem ntau yog siv nyob rau hauv cov chaw kuaj mob pathology thiab kev tshawb fawb qhov chaw uas yuav tsum tau kuaj histological ntawm cov ntaub so ntswg rau kev kuaj mob, kev tshawb fawb kab mob, thiab kev tshawb fawb lom neeg.

Cov ntaub so ntswg Embedder siv rau dab tsi?

Cov ntaub so ntswg embedders yog siv rau cov hom phiaj hauv qab no:

Histology

Cov ntaub so ntswg embedders feem ntau yog siv nyob rau hauv histology rau embed cov ntaub so ntswg lom nyob rau hauv ib tug haum nruab nrab, xws li paraffin wax, ua ntej sectioning thiab staining rau microscopic tsom xam.

Kev ruaj ntseg thiab tuav

Embedding cov ntaub so ntswg nyob rau hauv ib tug ruaj khov nruab nrab tso cai rau kom zoo preservation ntawm cov ntaub so ntswg qauv thiab yooj yim tuav thaum lub sij hawm cov txheej txheem histological.

Kev faib tawm

Embed cov ntaub so ntswg nyob rau hauv ib tug ruaj nruab nrab tsim ib tug thaiv uas yuav muab tau yooj yim sectioned siv ib tug microtome rau hauv nyias slices rau kev ntsuam xyuas nyob rau hauv ib tug microscope.

Staining thiab Visualization

Cov ntaub so ntswg embedded tuaj yeem stained nrog ntau yam dyes los txhim kho kev pom thiab kev sib txawv ntawm cov khoom siv cellular rau kev kuaj mob histopathological thiab kev tshawb fawb.

Cov ntawv thov tshawb fawb

Cov ntaub so ntswg embedders kuj tseem siv rau hauv kev tshawb fawb chaw rau kev kawm txog cell thiab cov ntaub so ntswg, kev hloov pauv pathological, thiab cov teebmeem ntawm cov tshuaj lossis kev kho mob ntawm cov ntaub so ntswg.

Kev kuaj mob

Hauv cov chaw kuaj mob pathology, cov ntaub so ntswg embedders yog siv rau kev npaj cov ntaub so ntswg kuaj tau los ntawm biopsies lossis phais rau kev kuaj mob thiab tshuaj xyuas.

Yuav ua li cas koj Embed ib cov ntaub so ntswg?

Embedding ib tug qauv ntaub so ntswg feem ntau muaj cov kauj ruam hauv qab no:

Kev npaj cov ntaub so ntswg:Freshly excised cov ntaub so ntswg yog sau thiab tam sim ntawd tsau nyob rau hauv ib tug fixative tov, xws li formaldehyde los yog paraformaldehyde, los khaws nws cov qauv.

Ntxuav:Cov ntaub so ntswg yog huv si ntxuav nrog dej kais dej kom tshem tawm cov tshaj fixative thiab impurities.

Lub cev qhuav dej:Cov ntaub so ntswg ntxuav yog lub cev qhuav dej siv cov kev daws teeb meem cawv, pib nrog 70% cawv thiab nce mus rau 95% thiab 100% cawv, kom tshem tawm cov dej seem.

Kev tshem tawm:Cov ntaub so ntswg uas lub cev qhuav dej yog tshem tawm ntawm cov cawv uas tseem tshuav siv cov tshuaj tshem tawm, xws li xylene lossis toluene.

Embedding Medium:Ib qho nruab nrab embedded, xws li paraffin wax, yog rhuab thiab yaj. Lub melted embedding nruab nrab yog tov nrog ib tug hardening neeg sawv cev, xws li beeswax, kom ua tau raws li qhov xav tau sib xws.

Cov ntaub so ntswg Placement:Lub cev qhuav dej thiab tshem tawm cov ntaub so ntswg yog muab tso rau hauv ib lub iav swb los yog hauv ib lub cassette tsim rau cov ntaub so ntswg embedded. Cov qauv ntaub so ntswg tau muab tso rau kom zoo kom ntseeg tau tias nws nyob hauv nruab nrab thiab tsis cuam tshuam thaum lub sij hawm embedding txheej txheem.

Npog nrog Embedding Medium:Lub melted embedding nruab nrab yog poured tshaj cov ntaub so ntswg qauv kom txog thaum nws yog kiag li submerged. Qhov nruab nrab embedding yog tso cai rau solidify nyob ib ncig ntawm cov ntaub so ntswg, tsim ib tug encasement los yog thaiv.

Tshem Tawm Tshaj Tawm:Thaum qhov nruab nrab embedding tau hardened, cov ntaub so ntswg thaiv yog ua tib zoo muab tshem tawm ntawm lub iav swb los yog cassette thiab ib tug tshaj embedding nruab nrab yog trimmed tam sim ntawd.

Storage thiab Sectioning:Cov ntaub so ntswg thaiv yog khaws cia rau hauv chav tsev kub kom txog thaum nws npaj txhij los muab faib siv microtome rau kev ua haujlwm histological ntxiv thiab tshuaj xyuas.

Cov kauj ruam tshwj xeeb thiab cov xwm txheej yuav txawv nyob ntawm seb cov ntaub so ntswg, cov txheej txheem kuaj, thiab cov kev cai tshwj xeeb ntawm cov txheej txheem kuaj mob. Kev tuav kom zoo thiab ua tib zoo ua txhua kauj ruam yog qhov tseem ceeb kom tau txais cov ntaub so ntswg zoo rau kev ntsuam xyuas histopathological kom raug.

Yam uas cuam tshuam cov ntaub so ntswg embedding

Muaj cov txheej txheem sib txawv uas siv los ntawm cov kws tshaj lij histotechnology, tab sis txawm tias txoj kev twg koj lub chaw sim siv, muaj cov ntsiab lus tseem ceeb uas tuaj yeem cuam tshuam rau qhov zoo ntawm embedding. Cov no suav nrog: cov qauv tuab, cov ntaub so ntswg orientation, zoo ntawm paraffin wax, pwm thiab cassette hom, thiab muaj lub teeb. Cia peb saib ze dua ntawm cov xwm txheej no:

Lub cev Orientation:

Kev taw qhia tsis raug yuav ua rau muaj kev tsis sib haum xeeb ntawm histological nta ntawm tus swb. Piv txwv li, thaum orienting ntawm daim tawv nqaij, cov qauv yuav tsum tau positioned kom lub epithelial npoo, cov ntaub so ntswg subcutaneous thiab tob txheej, yog raws li nyob rau hauv qab kom lub strata yuav pom nyob rau hauv lub tiav swb. Thaum embed ntau tshaj ib qho piv txwv, tag nrho cov ntaub so ntswg yuav tsum tau embedded nrees rau hauv qab ntawm lub thawv kom cov ntu txiav yuav nthuav qhia siv tau cov ntaub so ntswg xa.

Cov khoom pov thawj vim qhov kev taw qhia tsis raug yog feem ntau ntsib thaum cov txheej txheem embedding uas tuaj yeem ua rau kev puas tsuaj rau microtome, rhuav tshem ntawm ntu yog li ua rau kev tshawb fawb microscopic ntawm cov ntaub so ntswg nyuaj. Qhov tshwm sim yog qhov yuav tsum tau rov ua dua ua rau lub sijhawm hloov pauv ntev (TAT).

Paraffin Wax Quality/Composition:

Paraffin wax yog ib qho tseem ceeb tshaj plaws ntawm cov ntaub so ntswg embedding thiab nws qhov zoo yog qhov tseem ceeb rau kev tsim cov block. Paraffin wax formulations yuav tsum tau tsim nrog zoo ib yam thiab kev cia siab rau txo cov ntaub so ntswg distortion thiab muab tshwj xeeb compression kuj thiab ribbon continuity. Cov paraffins sib txawv muaj ntau yam ntawm qhov kub thiab txias, cov khoom siv ntxiv, thiab cov yam ntxwv, muaj paraffin kom tau raws li koj cov kev xav tau, embedding, thiab sectioning xav tau - yog li xav txog koj cov kev xav tau ua tib zoo thiab ua koj cov kev tshawb fawb.

Piv txwv li, Paraplast Regular yog pom zoo rau cov ntaub so ntswg embedding. Nws yog tsim los ntawm kev sib xyaw ua kom zoo ntawm cov purified paraffin uas muaj cov yas polymers ntawm kev tswj qhov hnyav molecular. Qhov no txhawb kev txhim kho cov ntaub so ntswg infiltration thiab superior zoo seem nrog tsawg compression.

Ib tug histotech kuj yuav ntsib impurities. Cov impurities xws li dawb floccus yuav tsum tsis txhob tshwm sim. Lub xub ntiag ntawm dawb floccus qhia tau hais tias tshem tawm ntau tshaj nyob rau hauv paraffin. Txhawm rau zam qhov no, nce qhov zaus uas koj hloov cov paraffin. Dhau li; Nws raug nquahu kom siv lub bolt zoo li cov khoom siv los ua cov ntaub so ntswg kom tiaj tus li ua tau kom sib npaug los ntawm cov hniav thaum microtomy

Pwm Loj thiab Cassette Hom:

Txawm hais tias tsis yog ib qho teeb meem tshwm sim, kev paub txog kev muaj ntau qhov sib txawv ntawm pwm los ntawm tus kws kho mob lossis tus kws kho mob tus kws kho mob (PA) yog qhov tseem ceeb kom nkag siab ua ntej los npaj qhov loj ntawm cov ntaub so ntswg xaiv rau kev tsom xam (saib cov qauv tuab), thiab tib yam yog muaj tseeb rau kev xaiv cov cassettes rau cov ntaub so ntswg kom ua tiav. Qee cov cassettes muaj qhov me me uas ua rau nyuaj kom dhau qhov nruab nrab embedding kom dhau los ua cov ntaub so ntswg. Lwm tus muaj qhov loj heev, vim li ntawd cov ntaub so ntswg me me lossis cov khoom tawg tuaj yeem ploj thaum ua cov ntaub so ntswg.

Muaj Lub Teeb:

Cov ntaub so ntswg me me tuaj yeem ploj ntawm sab laug tsis muaj kev kos vim tias muaj peev xwm rau cov kws kuaj mob histology pom thaum embedded. Tias yog vim li cas niaj hnub embedding chaw muaj nyob nrog tshem tau / tsis tshem tau lub iav magnifying. Qhov chaw ua haujlwm zoo teeb pom kev zoo kuj tseem ceeb heev thiab cov chaw embedding thiab coj teeb kuj muaj.

Sample Thickness:

Thaum sau tag nrho cov ntaub so ntswg yuav thicker los yog loj dua lub peev xwm ntawm cov pwm siv rau embedding ntawm cov ntaub so ntswg. Qhov no tuaj yeem tshwm sim yog tias tus kws kho mob lossis tus kws kho mob pathology tsis paub txog cov pwm loj npaum li cas tau siv hauv chav kuaj histology. Txhawm rau txhim kho qhov teeb meem no, cov ntaub so ntswg tuaj yeem muab faib thiab muab tso rau hauv ntau qhov thaiv, tab sis yuav tsum paub zoo thiab kev nkag siab ntawm cov ntaub so ntswg yog qhov tseem ceeb thiab qhov no tsuas yog yuav tsum tau ua los ntawm cov kws paub txog kev kuaj mob histology xwb. Yog tias koj tsis paub meej, sab laj nrog tus kws tshaj lij tech. Nws tseem yog ib qho tseem ceeb los tham txog qhov teeb meem no rau koj tus kws kho mob lossis PA kom tsis txhob muaj teeb meem yav tom ntej.

Cov ntaub ntawv dab tsi yog siv rau Embed Tissue?

Ntxiv nrog rau paraffin wax, lwm yam kev tshaj tawm xws li cov seem khov (xws li, OCT compound) tuaj yeem siv rau cov ntawv thov tshwj xeeb xws li immunohistochemistry lossis hauv cov chaw tshawb fawb uas yuav tsum tau khov sai. Txawm li cas los xij, rau niaj hnub histopathology, paraffin wax tseem yog tus qauv embedding khoom vim nws cov khoom tsim tau zoo thiab siv dav hauv cov txheej txheem kuaj mob.Cov khoom siv feem ntau siv los kos cov ntaub so ntswg kuaj rau kev ua histological yog paraffin wax. Paraffin wax yog cov khoom siv dawb los yog daj daj tau los ntawm cov roj av uas tau siv dav hauv histology vim nws muaj ntau yam thiab sib haum nrog ntau yam kev siv tshuaj pleev xim.Paraffin wax muaj ntau yam zoo li cov khoom siv embedding:

Inertness:Paraffin wax yog chemically inert thiab tsis hnov mob nrog cov ntaub so ntswg, khaws cia cov morphology thiab tso cai rau kev txhais lus histopathological kom raug.

Malleability:Paraffin wax tuaj yeem ua tau yooj yim molded thiab zoo li tus, ua rau kev tsim cov khoom sib xws nyob ib ncig ntawm cov ntaub so ntswg.

Sectionability:Thaum solidified, paraffin wax tsim ib qho kev ruaj khov uas tuaj yeem yooj yim faib rau hauv nyias slices siv microtome.

Compatibility:Paraffin wax yog sib xws nrog ntau yam histological stains, tso cai rau cov ncauj lus kom ntxaws pom ntawm cellular qauv thiab kev hloov pauv pathological.

Muaj thiab Nqi:Paraffin wax tau yooj yim muaj, pheej yig, thiab yooj yim rau kev ua haujlwm nrog, ua rau nws yog cov khoom xaiv rau embedding cov ntaub so ntswg hauv cov chaw kuaj mob histopathology thoob ntiaj teb.

Dab tsi yog Qhov Zoo Tshaj Plaws Nruab Nrab rau Kev Ua Npuas Ncauj?

Txawm hais tias lwm cov xov xwm embedding xws li cov seem khov (piv txwv li, OCT compound) yuav siv tau rau cov ntawv thov tshwj xeeb xws li immunohistochemistry lossis hauv kev tshawb fawb qhov chaw uas yuav tsum tau khov ceev ceev, paraffin wax tseem yog cov txheej txheem embedding khoom rau niaj hnub histopathology thiab cov txheej txheem kuaj mob vim nws cov khoom tsim. thiab siv dav. Rau niaj hnub histopathology thiab kuaj lub hom phiaj, paraffin wax yog suav tias yog qhov zoo tshaj plaws embedding nruab nrab rau cov ntaub so ntswg ua vim yog cov hauv qab no:

Inertness

Paraffin wax tsis hnov mob nrog cov ntaub so ntswg, khaws cia morphology thiab tso cai rau kev txhais lus histopathological kom raug.

Malleability

Paraffin wax tuaj yeem ua tau yooj yim molded thiab zoo li tus, ua rau kev tsim cov khoom sib xws nyob ib ncig ntawm cov ntaub so ntswg.

Sectionability

Thaum solidified, paraffin wax tsim ib qho kev ruaj khov uas tuaj yeem yooj yim faib rau hauv nyias slices siv microtome.

Kev sib haum xeeb

Paraffin wax yog sib xws nrog ntau yam histological stains, tso cai rau cov ncauj lus kom ntxaws pom ntawm cellular qauv thiab kev hloov pauv pathological.

Muaj thiab Nqi

Paraffin wax tau yooj yim muaj, pheej yig, thiab yooj yim rau kev ua haujlwm nrog, ua rau nws yog cov khoom xaiv rau embedding cov ntaub so ntswg hauv cov chaw kuaj mob histopathology thoob ntiaj teb.

Yuav xa cov ntaub so ntswg rau Embedding li cas

Paraffin Embedding

Cov ntaub so ntswg tuaj yeem lees txais ntau txoj hauv kev thiab cov nqi raug kho raws li cov txheej txheem no.
Cov ntaub so ntswg tshiab:Yog tias cov ntaub so ntswg tshiab raug xa mus, nws yuav muab tso rau hauv 10% Neutral Buffered Formalin rau kev kho thaum hmo ntuj ua ntej ua haujlwm.
Cov ntaub so ntswg ruaj khov:Peb txais cov ntaub so ntswg uas tau kho nyob rau hauv ntau yam fixatives. Thaum kho tiav, thov hloov cov qauv mus rau 70% Ethanol.
Kho cov ntaub so ntswg hauv Cassettes:Cov neeg siv khoom zoo siab tos txais muab lawv cov ntaub so ntswg tso rau hauv cov cassettes kom ntseeg tau tias xav tau kev taw qhia.

Cov hauv qab no muaj rau peb cov neeg siv khoom siv thiab muaj rau tuaj tos hauv E1515:
10% Neutral Buffered Formalin (NBF)
Cassettes (rau tus nqi)
Biopsy wraps/cassettes

Npaj cassettes

Kho cov ntaub so ntswg raws li xav tau:Tso cai rau ~ 1 mm / teev rau fixative nkag mus rau koj cov ntaub so ntswg thiab ib tug ntim ntawm 10-20 lub sij hawm fixative ntim rau cov ntaub so ntswg.
Daim ntawv lo cassettes:Thaum sau cov ntawv cassettes, tsuas yog siv cov cim reagent resistive (piv txwv li: Statmark Pen) lossis tus cwj mem # 2 nyuaj rau cov cassettes, tsis txhob siv tus cwj mem lossis Sharpie cim. Cov kuab tshuaj siv hauv kev ua haujlwm tuaj yeem tshem tawm cov number case los ntawm ntau cov cim "sawv cev" Sharpie.
Xyuas kom cov khoom me me yuav tsis ploj:Txhawm rau tiv thaiv cov ntaub so ntswg me me los ntawm kev ploj thaum ua tiav, muab tso rau hauv biopsy cassettes lossis qhwv hauv ntawv lim. Cov ntaub so ntswg processor siv lub tshuab nqus tsev los ua kom yooj yim infiltration uas tuaj yeem tshem tawm cov ntaub so ntswg ntawm lub cassette.
Muab tso rau hauv cassettes nyob rau hauv xav orientation:Thaum cov ntaub so ntswg muab tso rau hauv cassettes cov ntaub so ntswg nto uas muab tso rau hauv lub cassette yuav muab tso rau embedding thiab qhov no yog qhov chaw uas yog sectioned ua ntej. Thov sau ib qho tshwj xeeb embedding thov rau ntawm daim ntawv xa.
Muab cov khoom pov tseg rau hauv lub thawv ru tsev nrog lub hau nruj nruj. Nco ntsoov tias tag nrho cov ntaub so ntswg thiab los yog cassettes yog tag nrho submerged nyob rau hauv 70% Ethanol yog li cov qauv tsis qhuav tawm.
Sau lub thawv thauj khoom:Thov suav nrog lub npe ntawm PI, lub npe ntawm Tus Kws Tshawb Fawb (submitter), kev daws teeb meem, thiab hnub tim.
Muab cov qauv thiab daim ntawv xa mus rau RHS. Ib daim ntawv cais kuj tau muab los sau cov qauv qhia.

Frozen Samples

Cov ntaub so ntswg tuaj yeem lees txais ntau txoj hauv kev thiab cov nqi raug kho raws li cov txheej txheem no.
Kev nrawm nrawm dua li maj mam khov txo cov dej khov siv lead ua hauv cov ntaub so ntswg.
Cov ntaub so ntswg tshiab:Yog hais tias cov ntaub so ntswg tshiab raug xa mus, ceev ceev khov yam tsis muaj kev kho.
Cov ntaub so ntswg ruaj khov:Peb txais cov ntaub so ntswg uas tau kho nyob rau hauv ntau yam fixatives. Qee cov neeg siv khoom xaiv los npaj lawv cov qauv los ntawm sucrose gradient ua ntej khov. Thaum cov ntaub so ntswg tau npaj, thov hloov cov qauv mus rau 30% sucrose ua ntej xa mus rau RHS.
Frozen blocks hauv OCT pwm: Cov neeg siv khoom tau txais tos kom khov lawv tus kheej cov qauv kom ntseeg tau tias xav tau kev taw qhia. Frozen blocks tuaj yeem muab rau RHS rau kev faib / ua haujlwm ntxiv.

Txhawm rau OCT embed koj cov ntaub so ntswg

Ib daim ntawv lo pwm ua ntej tau sau ib feem peb nrog OCT embedding compound.
Cov ntaub so ntswg yuav tsum tau maj mam blotted dawb ntawm extraneous kua ua ntej yuav khov.
Cov ntaub so ntswg yog taw qhia hauv cov ntaub so ntswg pwm (kev tshem tawm ntawm ntug ntawm cov ntaub so ntswg thiab sab ntawm pwm yuav tsum tau khaws cia)
Cov seem ntawm cov ntaub so ntswg yog sau nrog OCT embedding nruab nrab. Xyuas kom meej tias cov qauv yog ib puag ncig thiab npog los ntawm OCT.
Cov ntaub so ntswg yog ntab rau ntawm cov kua nitrogen da dej kom txog thaum khov tag. OCT yuav ruaj khov thiab opaque / dawb.
Tshem cov pwm los ntawm cov kua nitrogen thiab nruj nreem qhwv hauv ntawv ci.
Muab cov ntawv qhwv cov khoom ntim rau hauv lub hnab yas uas tau sau zoo nrog cov qauv qhia thiab hnub tim. Thov suav nrog lub npe ntawm PI, lub npe ntawm Tus Kws Tshawb Fawb (submitter), kev daws teeb meem, thiab hnub tim.
Cov ntaub so ntswg yuav tsum khaws cia ntawm -80 C thiab xa mus rau RHS ntawm cov dej khov qhuav.
Muab cov qauv thiab daim ntawv xa mus rau RHS. Ib daim ntawv cais kuj tau muab los sau cov qauv qhia.

Yuav ua li cas koj Embed daim tawv nqaij?

Nws yog ib qho tseem ceeb uas yuav tsum ua raws li cov txheej txheem kuaj xyuas kom raug thiab cov lus qhia kev nyab xeeb thaum tuav cov qauv tshuaj lom neeg thiab cov tshuaj uas koom nrog hauv cov txheej txheem ntawm cov ntaub so ntswg. Kev cob qhia kom raug thiab kev saib xyuas kuj tseem ceeb los xyuas kom meej thiab txhim khu kev qha.Embeding daim tawv nqaij feem ntau ua rau kev kuaj mob histological thiab koom nrog cov kauj ruam hauv qab no:

Sau thiab Fixation

Cov qauv ntawm daim tawv nqaij tshiab feem ntau yog sau thaum biopsies lossis phais. Cov ntaub so ntswg raug kho tam sim ntawd hauv cov tshuaj xws li formaldehyde lossis paraformaldehyde kom khaws lawv cov qauv thiab tiv thaiv kev lwj.

Lub cev qhuav dej

Tom qab kho, cov ntaub so ntswg yog dehydrated los ntawm kev ntxuav lawv nyob rau hauv ib tug series ntawm graded cawv daws, nce los ntawm qis mus rau siab dua feem pua (xws li, 70%, 90%, thiab thaum kawg 100%), kom tshem tawm cov dej seem.

Clearing

Cov ntaub so ntswg ntawm daim tawv nqaij qhuav yog tom qab ntawd tshem tawm cov cawv seem uas siv cov tshuaj tshem tawm, xws li xylene lossis toluene.

Ciab Embedding

Ib qho nruab nrab embedded, xws li paraffin wax, yog melted thiab rhuab. Lub melted ciab yog tov nrog ib tug hardening neeg sawv cev, zoo li beeswax, kom ua tau raws li qhov xav tau sib xws. Cov ntaub so ntswg tshem tawm yog muab tso rau hauv ib lub iav swb los yog hauv ib lub cassette tsim rau cov ntaub so ntswg embedded.

Npog nrog Wax

Lub melted ciab yog poured tshaj cov ntaub so ntswg qauv kom txog rau thaum nws yog kiag li submerged. Cov quav ciab yog tom qab ntawd tso cai rau solidify nyob ib ncig ntawm cov ntaub so ntswg, tsim ib lub hnab los yog thaiv.

Trimming thiab Sectioning

Thaum cov wax hardened, cov ntaub so ntswg thaiv yog ua tib zoo tshem tawm ntawm lub iav swb los yog cassette thiab tej wax ntau tshaj yog trimmed tam sim ntawd siv ib rab chais hniav los yog microtome hniav. Lub thaiv yog ces mounted mus rau ib tug microtome, uas yog ib tug tshwj xeeb ntsuas siv los txiav tsis tshua muaj nyias seem ntawm embedded ntaub so ntswg.

Kev npaj swb

Cov ntu nyias nyias raug txiav los ntawm qhov thaiv thiab xa mus rau hauv lub tshuab hluav taws xob, qhov chaw uas lawv tau stained nrog qhov tsim nyog histological stains los txhim kho qhov pom ntawm cov qauv ntawm tes thiab cov khoom.

Microscopic Examination

Tom qab ntawd cov ntaub so ntswg stained raug tshuaj xyuas nyob rau hauv lub tshuab kuaj kab mob los ntsuas qhov morphology, architecture, thiab txhua yam kev hloov pauv hauv cov ntaub so ntswg ntawm daim tawv nqaij.

Peb lub Hoobkas

Xyoo 2015, Kuohai tau lees paub tias yog "National High-Tech Enterprise." "Kuohai Medical Technology" muaj tsib lub tuam txhab, suav nrog Hubei Xiaogan Kuohai Medical Technology Co., Ltd., Xiaogan Kuohai Medical Technology Co., Ltd., Hubei Haishi Industrial Co., Ltd., Xiaogan Ruifeng Electronic Technology Co., Ltd., thiab Xiaogan Dinghang Decoration Engineering Co., Ltd. Cov khoom lag luam suav nrog kev lag luam xws li cov cuab yeej kho mob, biotechnology, hluav taws xob thev naus laus zis, cov khoom siv hauv tsev siab, thiab kho kom zoo nkauj engineering. Lub tuam txhab tsis tu ncua mus rau kev lag luam pab pawg tsim kho.

FAQ

Q: Cov ntaub so ntswg embedding siv rau dab tsi?

A: Cov ntaub so ntswg embedding feem ntau yog siv nyob rau hauv cov chaw kuaj mob histology rau kev npaj cov ntaub so ntswg kuaj ua ntej kuaj microscopic. Nws tso cai rau nyias ntu ntawm cov ntaub so ntswg txiav thiab stained, ua rau pom kev thiab tsom xam ntawm cov qauv cellular thiab cov kev hloov pauv pathological.

Q: Dab tsi ntawm cov ntaub so ntswg tuaj yeem kos tau?

A: Ntau yam ntaub so ntswg tuaj yeem muab tso rau, suav nrog tab sis tsis txwv rau ntawm daim tawv nqaij, nqaij, pob txha, daim siab, raum, thiab cov ntaub so ntswg. Cov txheej txheem no siv tau rau ob qho tib si tshiab thiab formalin-taw, paraffin-embedded (FFPE) cov ntaub so ntswg.

Q: Lub hom phiaj ntawm embedding cov ntaub so ntswg nyob rau hauv paraffin wax yog dab tsi?

A: Paraffin wax feem ntau yog siv los ua cov khoom nruab nrab vim nws tso cai rau kev tsim cov ntu nyias ntawm cov ntaub so ntswg (feem ntau nyob ib ncig ntawm 4-5} micrometers tuab) siv microtome. Cov quav ciab pab tswj cov txheej txheem kev ncaj ncees ntawm cov ntaub so ntswg thaum muab faib thiab muab lub hauv paus rau daim ntawv thov ntawm histological stains.

Q: Ntev npaum li cas cov ntaub so ntswg embedding siv sijhawm?

A: Lub sijhawm ntawm cov txheej txheem ntawm cov ntaub so ntswg embedding nyob ntawm ntau yam, nrog rau hom ntaub so ntswg, qhov loj ntawm cov qauv, thiab cov txheej txheem meej siv. Feem ntau, cov txheej txheem tuaj yeem siv sijhawm nyob qhov twg los ntawm ob peb teev mus rau ib hmos kom tiav lub cev qhuav dej, tshem tawm, thiab siv quav ciab ntawm cov ntaub so ntswg.

Q: Dab tsi yog qee qhov teeb meem tshwm sim thaum siv cov ntaub so ntswg?

A: Qee qhov kev sib tw thaum lub sij hawm cov ntaub so ntswg embedding tuaj yeem muaj xws li cov ntaub so ntswg puas los yog distortion thaum lub sij hawm lub cev qhuav dej los yog siv quav ciab pouring kauj ruam, tsis txaus fixation ua rau cov ntaub so ntswg tsis zoo, thiab teeb meem nrog rau qhov zoo ntawm lub embedding nruab nrab nws tus kheej. Kev cob qhia kom raug thiab kev paub txog kev tuav cov qauv ntaub so ntswg yog qhov tseem ceeb los txo cov teeb meem no.

Q: Yuav ua li cas yog cov ntaub so ntswg embedding txawv ntawm khov sectioning?

A: Frozen sectioning yog ib qho txheej txheem uas cov ntaub so ntswg tshiab tau khov sai, ntu siv lub cryostat, thiab tshuaj xyuas ncaj qha rau hauv lub tshuab tsom iav yam tsis tas yuav muaj qhov staining. Nyob rau hauv sib piv, cov ntaub so ntswg embedding muaj fixation, lub cev qhuav dej, ciab embedding, thiab sectioning nrog ib tug microtome, ua raws li los ntawm staining rau cov ncauj lus kom ntxaws tsom xam. Frozen sectioning feem ntau yog siv rau kev kuaj mob sai, thaum cov ntaub so ntswg embedding zoo dua rau kev tshawb fawb dav.

Q: Cov ntaub so ntswg puas tuaj yeem siv tau?

A: Yog lawm, ntau lub chaw kuaj histology niaj hnub siv cov ntaub so ntswg embedding uas tuaj yeem ua ntau yam qauv ib txhij, automating cov kauj ruam ntawm lub cev qhuav dej, tshem tawm, thiab siv quav ciab embedding. Cov tshuab no muab cov txiaj ntsig zoo xws li kev txhim kho kev tsim tawm, nce kev nkag mus, thiab txo qhov peev xwm rau tib neeg yuam kev.

Q: Puas muaj kev xav txog kev nyab xeeb thaum ua cov ntaub so ntswg?

A: Yog lawm. Kev tuav cov tshuaj xws li cawv, xylene, thiab paraffin wax yuav tsum tau ua raws li cov txheej txheem kev nyab xeeb hauv chav kuaj nruj. Kev ua pa kom raug, cov khoom siv tiv thaiv tus kheej (PPE) xws li hnab looj tes, hnab looj tes, thiab kev tiv thaiv qhov muag, thiab ceev faj tuav cov khoom uas muaj hluav taws kub yog txhua yam tseem ceeb uas yuav tsum tau xav txog. Tsis tas li ntawd, kev ua haujlwm nrog cov qauv tshuaj lom neeg muaj feem cuam tshuam rau cov khoom sib kis, yog li kev ntsuas biosafety tsim nyog yuav tsum muaj nyob rau hauv qhov chaw.

Q: Dab tsi yog qhov zoo ntawm cov ntaub so ntswg embedding?

A: Nws txo qis cov ntaub so ntswg thaum lub sijhawm ua haujlwm hnyav, tso cai rau ntau cov ntaub so ntswg me me hauv ib qho thaiv, thiab muab kev taw qhia rau kev faib tawm.

Q: Vim li cas cov ntaub so ntswg yuav tsum tau muab tso rau hauv ciab?

A: Embedding nyob rau hauv paraffin wax ua rau nyias ntu kom raug txiav thiab cov qauv ntawm cov ntaub so ntswg los soj ntsuam siv cov dyes yooj yim, xws li hematoxylin thiab eosin, kom delineate ntau yam ntawm tes.

Q: Dab tsi yog qhov zoo ntawm kev ua cov ntaub so ntswg?

A: Cov cuab yeej siv tau cov ntaub so ntswg ntau dua, ua haujlwm sai dua thiab ua kom tau txais txiaj ntsig zoo dua. Qhov zoo ntawm cov ntaub so ntswg tshuab ua haujlwm - txuag lub sijhawm, txo tib neeg kev ua yuam kev, ua kom cov dej ntws zoo, qhov kub thiab txias tuaj yeem hloov kho thiab lub tshuab nqus tsev / lub siab kuj tuaj yeem koom ua ke.

Q: Lub hom phiaj ntawm cov txheej txheem embedding yog dab tsi?

A: Embedding yog txheej txheem uas cov ntaub so ntswg los yog cov qauv raug kaw nyob rau hauv ib qho loj ntawm qhov nruab nrab embedding siv lub pwm. Txij li thaum cov ntaub so ntswg nyias nyias nyob rau hauv thickness lawv xav tau ib tug txhawb nruab nrab nyob rau hauv uas cov ntaub so ntswg blocks yog embedded. Qhov nruab nrab txhawb nqa no yog hu ua embedding nruab nrab.

Q: 3 qhov zoo ntawm cov ntaub so ntswg kab lis kev cai yog dab tsi?

A: Hauv qab no yog ntau yam zoo ntawm cov ntaub so ntswg kab lis kev cai: cov nroj tsuag tau txais nyob rau hauv ib lub sij hawm luv luv nrog ib tug me me ntawm cov ntaub so ntswg. Cov nroj tsuag tshiab tsim tawm tsis muaj kab mob. Cov nroj tsuag tuaj yeem loj hlob thoob plaws hauv lub xyoo, tsis hais lub caij nyoog.

Q: Dab tsi yog cov ntaub so ntswg embedder?

A: Cov ntaub so ntswg embedded. Cov txheej txheem ntawm kev tso cov hlwb los yog cov ntaub so ntswg rau hauv qhov nruab nrab txhawb kom nyias ntu tuaj yeem raug txiav siv microtome. Qhov nruab nrab tuaj yeem yog paraffin wax (paraffin embedding) lossis plastics (yas embedding) xws li epoxy resins.

Q: Dab tsi yog qhov zoo ntawm paraffin wax embedding?

A: Paraffin wax yog xaiv rau cov ntaub so ntswg embedding vim hais tias nws muab kev txhawb nqa rau txiav nyias seem thiab tso cai rau lub sij hawm ntev cia nyob rau hauv chav tsev kub. Paraffin wax feem ntau yog siv rau cov ntaub so ntswg embedding vim nws niaj hnub muaj thiab muaj peev xwm khaws cov ntaub so ntswg kev ncaj ncees thiab staining yam ntxwv.

Q: Dab tsi tshwm sim hauv cov ntaub so ntswg embedding?

A: Peb embed cov ntaub so ntswg ua qauv siv Histostar Tissue Embedding System. Cov ntaub so ntswg ua tiav yog taw qhia nyob rau hauv cov pwm hlau uas tom qab ntawd tau ntim nrog molten paraffin wax. Tom qab ntawd ib daim ntawv lo rau ntawm lub cassette yog muab tso rau saum thiab tseem ntim nrog quav ciab.

Q: Dab tsi yog qhov zoo ntawm cov ntaub so ntswg khov dua embedding?

A: Cov ntaub so ntswg khov ua rau muaj kev hloov pauv tsawg dua rau cov epitopes thiab yog li ntawd tuaj yeem muab cov yam ntxwv zoo dua qub piv rau cov txheej txheem raws li paraffin embedding.

Q: Qhov twg yog embedding siv?

A: Kev kos yog siv hauv kev txheeb xyuas cov ntawv. Feem ntau, tus sawv cev yog ib qho vector uas muaj txiaj ntsig tiag tiag uas encodes lub ntsiab lus ntawm lo lus hauv txoj hauv kev uas cov lus uas nyob ze rau hauv qhov chaw vector yuav tsum zoo sib xws hauv lub ntsiab lus.

Q: Hom kev embedding twg yog qhov nrov tshaj plaws?

A: Paraffin. Qhov feem ntau siv embedding nruab nrab rau kev siv tshuaj lom neeg yog paraffin wax, los yog 'paraffin. ' Paraffin wax yog insoluble hauv dej tab sis dissolves nyob rau hauv toluene thiab xylene thiab melts ntawm 46-68 degree, ua rau nws yog ib qho zoo tagnrho nruab nrab rau embedding cov ntaub so ntswg tom qab lawv tau qhuav dej.

Q: Yuav ua li cas tom qab embedded?

A: Kev faib tawm. Thaum cov ntaub so ntswg tau embedded, lawv yuav tsum tau txiav mus rau hauv seem uas yuav muab tso rau hauv ib tug swb. Qhov no yog ua tiav nrog microtome. Lub microtome yog tsis muaj dab tsi ntau tshaj li riam nrog ib tug txheej txheem rau kev ua kom ib tug paraffin thaiv tus qauv kev ncua deb ntawm nws.

Raws li ib qho ntawm cov ua cov ntaub so ntswg embedder manufacturers nyob rau hauv Suav teb, peb sov siab txais tos koj mus yuav cov nqi-npaum cov ntaub so ntswg embedder muag ntawm no los ntawm peb lub hoobkas. Tag nrho cov khoom customized yog nrog cov khoom zoo thiab cov nqi sib tw.